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    Biotin-16-Aminoallyl-2'-dCTP - (N-5002)

    2021-11-15
    浏览次数: 99

    Biotin-16-Aminoallyl-2'-dCTP - (N-5002)又名Biotin-16-dCTP, Biotin-16-AA-dCTP。

    Biotin-16-Aminoallyl-2'-dCTP - (N-5002)


    产品描述

    Biotin-16-Aminoallyl-2'-deoxycytidine-5'-Triphosphate可用于通过PCR、切口平移、使用Klenow(exo-)DNA聚合酶的引物延伸和末端脱氧核苷酸转移酶标记的3'端,将生物素掺入DNA中。可以使用Moloney鼠白血病病毒(M-MLV)逆转录酶与此dNTP制备生物素化的cDNA。


    产品信息

    货号

    N-5002

    规格

    50 nmole1 µmole

    纯度

    ≥90% by AX-HPLC

    保存温度

    -20℃或以下

    分子式

    C32H53N8O17P3S (free acid)

    盐离子形式

    Li+

    消光系数

    5,040 Lmol-1cm-1 at 290 nm

    溶液

    H2O

    浓度

    5 mM

    骨架

    5'-三磷酸盐

    糖类型

    DNA

    核苷酸类别

    生物素标记

    应用

    适体、表观遗传学/DNA损伤、体外转录、诱变、光交联研究


    文献参考:


    Green, Nathaniel M.; Moody, Krishna-Sulayman; Debatis, Michelle; Marshak-Rothstein, Ann . Activation of autoreactive B cells by endogenous TLR7 and TLR3 RNA ligands.


    Limbo, Oliver; Moiani, Davide; Kertokalio, Aryandi; Wyman, Claire; Tainer, John A.; Russell, Paul . Mre11 ATLD17/18 mutation retains Tel1/ATM activity but blocks DNA double-strand break repair.


    Song, Chun-Xiao; Diao, Jiajie; Brunger, Axel T.; Quake, Stephen R. . Simultaneous single-molecule epigenetic imaging of DNA methylation and hydroxymethylation.


    Gangras, Pooja; Dayeh, Daniel M.; Mabin, Justin W.; Nakanishi, Kotaro; Singh, Guramrit . Cloning and Identification of Recombinant Argonaute-Bound Small RNAs Using Next-Generation Sequencing.


    Hede, Marianne Smedegaard; Fjelstrup, Søren; Lötsch, Felix; Zoleko, Rella Manego; Klicpera, Anna; Groger, Mirjam; Mischlinger, Johannes; Endame, Lilian; Veletzky, Luzia; Neher, Ronja; Simonsen, Anne Katrine Wrist; Petersen, Eskild; Mombo-Ngoma, Ghyslain; Stougaard, Magnus; Ho, Yi-Ping; Labouriau, Rodrigo; Ramharter, Michael; Knudsen, Birgitta Ruth . Detection of the Malaria causing Plasmodium Parasite in Saliva from Infected Patients using Topoisomerase I Activity as a Biomarker.


    Veneziano, Rémi; Shepherd, Tyson R.; Ratanalert, Sakul; Bellou, Leila; Tao, Chaoqun; Bathe, Mark . In vitro synthesis of gene-length single-stranded DNA.


    Braun, Joerg E.; Friedman, Larry J.; Gelles, Jeff; Moore, Melissa J. . Synergistic assembly of human pre-spliceosomes across introns and exons.


    Ho, Nicholas R. Y.; Lim, Geok Soon; Sundah, Noah R.; Lim, Diana; Loh, Tze Ping; Shao, Huilin . Visual and modular detection of pathogen nucleic acids with enzyme-DNA molecular complexes.


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