Epicypher新品 | 核酸提取缓冲液
CUTANA™ 细胞核提取缓冲液是CUT&RUN和CUT&Tag实验中，用于从培养的细胞和组织中提取细胞核的必需试剂。这种缓冲液可与多种真核细胞和组织兼容。以下是使用该缓冲液成功进行细胞核分离的细胞和组织类型：
mouse NIH3T3 fibroblast cells | human A549 non-small cell lung cancer (NSCLC) cells |
human K562 leukemia cells | human NCI-H1299 non-small cell lung cancer (NSCLS cells) |
human bone marrow derived macrophages | human TIG-1 fetal lung cells |
human monocyte derived macrophages | human LoVo colorectal cancer cells |
human MV-4-11 macrophage cells | human LNCaP prostate carcinoma cells |
human SUM149 triple negative breast cancer (TNBC) cells | human renal primary cells |
human GM24385 B-lymphocyte (aka HG002) cells | human peripheral blood mononuclear cells (PBMCs) |
human MCF7 breast cancer cells | human intestinal tissue |
human MDA-MB-231 breast cancer cells | human HEPM embryonic cells |
human SK-MEL-2 melanoma cells |
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只需将试剂盒中的Pre-Nuclei Extraction Buffer和亚精胺与蛋白酶抑制剂混合即可完成该试剂的制备。CUTANA™ 细胞核提取缓冲液针对CUT&RUN和CUT&Tag实验流程进行了优化，从而获得高质量的细胞核，助力您的基因组图谱分析。
试剂盒组成
组分 | 货号 |
CUTANA™ Pre-Nuclei Extraction Buffer | 21-1026a |
1 M Spermidine | 21-1026b |
保存条件
将Pre-Nuclei Extraction Buffer 4ºC储存, 1 M亚精胺-20ºC储存。
自收到之日起可稳定储存6个月。
验证数据
Figure 1: Nuclei Extraction
Nuclei were extracted from 3 different cell types: K562, NIH3T3, and LNCaP using the CUTANA™ Nuclei Extraction Protocol for CUT&RUN and CUT&Tag. Top 3 panels show cells before extraction. Starting cells are viable (bright white and round). Bottom 3 panels show nuclei extracted using the CUTANA™ Nuclei Extraction Buffer as indicated by positive Trypan Blue staining.
订购详情
货号 | 产品名称 | 规格 |
21-1026 | CUTANA™ Nuclei Extraction Buffer | 100 Reactions |
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