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Nucleosome, Recombinant Human, H3K4,K27me3 dNuc, Biotinylated
品牌：EpiCypher
货号：16-0403 规格：50 µg 目录价：￥6900
产品详情
产品名称:
Nucleosome, Recombinant Human, H3K4,K27me3 dNuc, Biotinylated
产品描述:
Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P20671; H2B-O60814; H3.2-Q71DI3; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H3 (created by a proprietary semi-synthetic method) contains trimethylated-lysines at positions 4 and 27. The nucleosome is the basic subunit of chromatin. The 147 bp 601 sequence, identified by Lowary and Widom [1], has high affinity for histone octamers and is useful for nucleosome assembly. The DNA contains a 5’ biotin-TEG group. * H3K4,K27me3 has a Cys to Ala substitution at position 110.
保存温度:
Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
运输温度:
Dry ice.
产品形式:
H3K4,K27me3 dNuc (27.2 µg protein weight, 50 µg DNA + protein) in 50.0 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. Molarity = 5.0 μM. MW = 199,935.2 Da.
产地:
美国
生产商:
EpiCypher
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