来源于Escherichia coli（E.coli）的片段DNA可以用作核酸酶靶向切割和释放（CUT&RUN）的实验标准化的spike-in对照。产品CUTANA™ E. coli Spike-in DNA含有足够的材料，可用于100-200个CUT&RUN样本（高丰度和低丰度靶标的范围）。

产品详情

保存温度: Stable for 2 years at -20°C from date of receipt. After resuspending, aliquots should be stored at -20°C.

运输温度: Room temp. Can go on frozen cold packs or dry ice.

产品形式: 100 ng lyophilized DNA. *NOTE: May not be visible.

验证数据

Figure 1: DNA gel data E. coli fragment size distribution. Lane 1: gDNA extracted from JM101 E.  coli cells (500 ng). Lane 2: Digested and purified CUTANA™ E. coli Spike-in  DNA (500 ng) resolved via 2% E-Gel™ EX Agarose Gel. Migration positions  of DNA molecular weight markers are indicated.

Figure 2: CUT&RUN sequencing data CUTANA™ E. coli Spike-in DNA (1.0 ng) was added to CUT&RUN samples using 500,000 K562 cells enriched  for a low abundance target (H3K4me3, EpiCypher 13-0041), a high abundance target (H3K27me3, EpiCypher  13-0030) and an IgG negative control (EpiCypher 13-0042). Total numbers of paired-end sequencing reads, reads  aligned to E. coli, and percentage of total sequencing reads aligned to E. coli spike-in DNA are shown. NOTE:  Spike-in DNA amount should be optimized by the end user with the goal of E. coli DNA comprising ~1% (0.2 -  5%) of total sequencing reads.

订购详情

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