Annexin V-FITC Apoptosis Detection Kit

Annexin V Apoptosis Detection Kit is based on the observation that soon after initiating apoptosis, cells translocate the membrane phosphatidylserine (PS) from the inner face of the plasma membrane to the cell surface. Once on the cell surface, PS can be easily detected by staining with a fluorescent conjugate of Annexin V, a protein that has a high affinity for PS. The one-step staining procedure takes only 5-15 minutes. Detection can be analyzed by flow cytometry or fluorescence microscopy. The kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining._x000D_ _x000D_
Detection method - Flow cytometry (Ex = 488 nm; Em = 530 nm) and fluorescence microscopy_x000D_
Sample type - Living cells (suspension and adherant)_x000D_
Species reactivity - Mammalian_x000D_
Applications - Detect early/middle stages of apoptosis; differentiate apoptosis from necrosis._x000D_ _x000D_

Features and Benefits_x000D_
Simple one step staining procedure in 5-15 minutes_x000D_
Fast and convenient_x000D_
Kit can differentiate apoptosis vs necrosis when performing both Annexin V-FITC and PI staining_x000D_ _x000D_

Kit Contents:_x000D_ _x000D_
Annexin V-FITC - Formulated in 0.01 M PBS pH 7.4, 150 mM NaCl, 1% BSA and 0.09% sodium azide as a preservative._x000D_
1X Binding Buffer - Formulated in 0.01 M PBS pH 7.4, 150 mM NaCl. (Caution: Propidium Iodide Solution is toxigenic and mutagenic; Please handle with care.)_x000D_
Propidium Iodide (PI) - No preservative added_x000D_ _x000D_

Other Materials and Solutions Required:_x000D_ _x000D_
Fluorescence Microscope or Flow Cytometer (Capable of FITC and Propidium Iodide Detection)_x000D_ _x000D_
Microcentrifuge_x000D_ _x000D_
Adjustable Pipettors (100-1000 μl, 10-200 μl, and 1-20 μl)_x000D_ _x000D_
Ice Bucket and Ice_x000D_ _x000D_
PBS Buffer_x000D_ _x000D_
Microcentrifuge Tubes

FACS, ICC/IF

Stable when stored dark at 2º-8ºC. Do Not Freeze.

4°C

美国