Epicypher新品 | 核酸提取缓冲液

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Epicypher新品 | 核酸提取缓冲液

发布日期：2024-09-18

浏览次数：11

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CUTANA™ 细胞核提取缓冲液是CUT&RUN和CUT&Tag实验中，用于从培养的细胞和组织中提取细胞核的必需试剂。这种缓冲液可与多种真核细胞和组织兼容。以下是使用该缓冲液成功进行细胞核分离的细胞和组织类型：

mouse NIH3T3 fibroblast cells	human A549 non-small cell lung cancer (NSCLC) cells
human K562 leukemia cells	human NCI-H1299 non-small cell lung cancer (NSCLS cells)
human bone marrow derived macrophages	human TIG-1 fetal lung cells
human monocyte derived macrophages	human LoVo colorectal cancer cells
human MV-4-11 macrophage cells	human LNCaP prostate carcinoma cells
human SUM149 triple negative breast cancer (TNBC) cells	human renal primary cells
human GM24385 B-lymphocyte (aka HG002) cells	human peripheral blood mononuclear cells (PBMCs)
human MCF7 breast cancer cells	human intestinal tissue
human MDA-MB-231 breast cancer cells	human HEPM embryonic cells
human SK-MEL-2 melanoma cells

只需将试剂盒中的Pre-Nuclei Extraction Buffer和亚精胺与蛋白酶抑制剂混合即可完成该试剂的制备。CUTANA™ 细胞核提取缓冲液针对CUT&RUN和CUT&Tag实验流程进行了优化，从而获得高质量的细胞核，助力您的基因组图谱分析。

试剂盒组成

组分	货号
CUTANA™ Pre-Nuclei Extraction Buffer	21-1026a
1 M Spermidine	21-1026b

保存条件

将Pre-Nuclei Extraction Buffer 4ºC储存, 1 M亚精胺-20ºC储存。

自收到之日起可稳定储存6个月。

验证数据

验证数据.png

Figure 1: Nuclei Extraction

Nuclei were extracted from 3 different cell types: K562, NIH3T3, and LNCaP using the CUTANA™ Nuclei Extraction Protocol for CUT&RUN and CUT&Tag. Top 3 panels show cells before extraction. Starting cells are viable (bright white and round). Bottom 3 panels show nuclei extracted using the CUTANA™ Nuclei Extraction Buffer as indicated by positive Trypan Blue staining.

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