AdipoGen新品推荐|NLRP3炎症小体抗体套装-精品推荐-ELISA_抗体_蛋白_核苷酸_试剂盒-深圳公海赌赌船官网登录网址科技有限公司

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AdipoGen新品推荐|NLRP3炎症小体抗体套装

发布日期：2024-10-15

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AdipoGen新品推荐|NLRP3炎症小体抗体套装

炎症小体是一种多蛋白复合物，其活性与生理性和病理性炎症有关，可激活炎症性Caspases并激活IL-1家族的细胞因子（IL-1β、IL-18 和 IL-1α）。目前已经报道了几种含有不同传感蛋白的炎症小体，如NLRP1、NLRP3、IPAF、NLRP6、NLRP10、NLRP12、Pyrin、RIG-I 和AIM-2。这些炎症小体大多需要适配蛋白ASC（凋亡相关斑点样蛋白，含CARD）招募Caspase-1，Caspase-1被裂解和激活，导致其各种靶点被裂解，并引起炎症因子 IL-1β和IL-18的成熟和分泌。

NLRP3/NALP3属于NOD样受体家族，连接刺激信号与Caspase-1激活，是炎症小体的重要组成部分，在炎症调控中发挥着关键作用。越来越多的证据表明NLRP3/NALP3的突变会导致一系列自身炎症性疾病，如家族性寒冷型自身炎症性综合征（FCAS）、穆-韦综合征(MWS)和慢性幼儿神经皮肤关节综合征（CINCA）。

检测炎症小体激活的金标准方法是炎症小体Caspase-1的免疫印迹，但也存在一定的技术难度，特别是在蛋白质沉淀、蛋白质分离和转移方案的选择方面。另外，炎症启动和激活步骤也是免疫印迹实验成功的关键。

AdipoGen推出了NLRP3炎症小体抗体套装，下面列出了抗体套装相关信息以及使用AdipoGen标准抗体检测炎症小体的方法，为您实验的顺利进行保驾护航！同时也请注意产品说明书中对NLRP3进行WB实验时的小tips！

产品货号	产品名称	产品组分	产品说明书
AG-44B-0008	NLRP3 Inflammasome Human Antibodies Starter Set	AG-20B-0014 anti-NLRP3/NALP3, mAb(Cryo-2)-25µg AG-20B-0048 anti-Caspase-1(p20)(human), mAb(Bally-1) - 25µg AG-25B-0006 anti-Asc, pAb(AL177)-25µg	https://adipogen.com/productmanagement/resource/download/type/document/file/AG-44B-0008_NLRP3_Inflammasome_Human_Antibodies_Starter_Set_v1_final.pdf
AG-44B-0009	NLRP3 Inflammasome Mouse Antibodies Starter Set	AG-20B-0014 anti-NLRP3/NALP3, mAb(Cryo-2)-25µg AG-20B-0042 anti-Caspase-1(p20)(mouse), mAb(Casper-1) - 25µg AG-25B-0006 anti-Asc, pAb(AL177)-25µg	https://adipogen.com/productmanagement/resource/download/type/document/file/AG-44B-0009_NLRP3_Inflammasome_Mouse_Antibodies_Starter_Set_v1_Final.pdf
AG-44B-0010	NLRP3 Inflammasome Human Reagents Starter Set	AG-20B-0014 anti-NLRP3/NALP3, mAb(Cryo-2)-25µg AG-20B-0048 anti-Caspase-1(p20)(human), mAb(Bally-1)-25µg AG-25B-0006 anti-Asc, pAb(AL177)-25µg IAX-100-007 LPS from E. coli R515(Re) TLRpure Sterile Solution-500µg AG-CN2-0020 Nigericin . sodium salt-5 mg	https://adipogen.com/productmanagement/resource/download/type/document/file/AG-44B-0010_NLRP3_Inflammasome_Human_Reagents_Starter_Set_v1.pdf
AG-44B-0011	NLRP3 Inflammasome Mouse Reagents Starter Set	AG-20B-0014 anti-NLRP3/NALP3, mAb(Cryo-2)-25µg AG-20B-0042 anti-Caspase-1(p20)(mouse), mAb(Casper-1)-25µg AG-25B-0006 anti-Asc, pAb(AL177)-25µg IAX-100-007 LPS from E. coli R515(Re) TLRpure Sterile Solution-500µg AG-CN2-0020 Nigericin . sodium salt-5 mg	https://adipogen.com/productmanagement/resource/download/type/document/file/AG-44B-0011_NLRP3_Inflammasome_Mouse_Reagents_Starter_Set_v1.pdf

相关产品：使用标准KO-extract的验证抗体——领域专家选择使用并发表文章!

产品货号	产品名称	Protocol
AG-20B-0042	anti-Caspase-1 (p20) (mouse), mAb (Casper-1)	1. Measuring the Inflammasome (Download Protocol) 2. Immunoblotting for Active Caspase-1 (Download Protocol) 3. Inflammasome Activation and Inhibition in Primary Murine Bone 4. Marrow-Derived Cells, and Assays for IL-1α, IL-1β, and Caspase-1 (Download Protocol) 5. Assessing Caspase-1 Activation (Download Protocol)
AG-20B-0044	anti-Caspase-1 (p10) (mouse), mAb (Casper-2)	1. Measuring the Inflammasome (Download Protocol)
AG-20B-0048	anti-Caspase-1 (p20) (human), mAb (Bally-1)	1. Measuring the Inflammasome (Download Protocol) 2. Cell-Free Assay for Inflammasome Activation (Download Protocol)
AG-20B-0014	anti-NLRP3/NALP3, mAb (Cryo-2)	1. Measuring NLR Oligomerization I: Size Exclusion Chromatography, Co-immunoprecipitation, and Cross-Linking (Download Protocol) 2. Functional Reconstruction of NLRs in HEK293 Cells (Download Protocol)
AG-20B-0084	anti-NLRP1b (mouse), mAb (2A12)	1. Functional degradation: a mechanism of NLRP1 inflammasome activation by diverse pathogen enzymes: A. Sandstrom, et al.; Science 364, 82 (2019) (Publication)
AG-25B-0006	anti-Asc, pAb (AL177)	1. Measuring Inflammasome Activation in Response to Bacterial Infection (Download Protocol) 2. Measuring NLR Oligomerization II: Detection of ASC Speck Formation by Confocal Microscopy and Immunofluorescence (Download Protocol) 3. Cell-Free Assay for Inflammasome Activation (Download Protocol) 4. Flow Cytometric Detection of AC Specks (Download Protocol)
AG-20B-0009	anti-RIG-I, mAb (Alme-1)	1. The Human Papillomavirus E6 Oncoprotein Targets USP15 and TRIM25 To Suppress RIG-I-Mediated Innate Immune Signaling: C. Chiang, et al.; J. Virol. 92, e01737-17 (2018) [KO Validation] (Publication)
AG-20B-0010	anti-ZBP1, mAb (Zippy-1)	1. Species-independent contribution of ZBP1/DAI/DLM-1-triggered necroptosis in host defense against HSV1: H. Guo, et al.; Cell Death Dis. 9, 816 (2018) [KO Validation] (Publication)