VECTASTAIN® Universal Quick HRP Kit, Peroxidase (Concentrate), 1 Kit

VECTASTAIN Universal Quick Peroxidase Staining Kit has more than 50,000 citations to its credit and remains widely popular. Based on the versatile avidin-biotin complex interaction, the system is modular, and along with our selection of secondary antibodies, can accommodate a wide array of primary antibody and tissue species. Our ABC kits are economical and continue to be a staple product in any immunohistochemistry (IHC) and immunocytochemistry (ICC) laboratory.Peroxidase substrates produce sharp, dense precipitates with crisp localization. These characteristics, in conjunction with the high sensitivity and low background of the VECTASTAIN® ABC systems, make the peroxidase enzyme a preferred choice in many applications. (eg. In neural tissue, the peroxidase system is often preferred because it gives more consistent labeling of both cell bodies and processes.)VECTASTAIN® Universal Quick KitsThe VECTASTAIN® Universal Quick Kit is designed for rapid immunohistochemical staining of tissue sections. The kit relies on a proprietary preformed peroxidase-streptavidin complex to achieve outstanding sensitivity with short incubation times.Rapid protocol: Staining in less than 20 minutes following primary antibody incubation. Working solutions can be used immediately after dilution.High sensitivity, low background.Biotinylated, Universal Pan-Specific secondary antibody recognizes mouse, rabbit, and goat primary antibodies as well as those from related species such as rat, bovine, and sheep.*Available in Ready-To-Use (R.T.U.) format: Prediluted, stabilized working solutions of VECTASTAIN® Universal Quick Kit reagents provide the same high sensitivity and low background as the concentrated VECTASTAIN® Universal Kit.*Do not use the pan-specific secondary antibody to stain rat, mouse or other rodent, rabbit, goat, bovine, or sheep tissue due to potential reactivity with endogenous IgG.Kit Contents:6 ml normal horse blocking serum2.2 ml biotinylated universal, pan-specific (anti-mouse/rabbit/goat IgG) antibody1.2 ml streptavidin peroxidase preformed complexSufficient reagents to stain approximately 250-500 sections. The Avidin Biotin Complex MethodThe VECTASTAIN® ABC systems are extremely sensitive due to the form and number of active enzyme molecules associated with the preformed Avidin/Biotinylated enzyme Complex. This ABC complex takes advantage of two important properties of avidin: 1) an extraordinarily high affinity for biotin (over one million times higher than antibody for most antigens), and 2) four biotin-binding sites. These properties allow macromolecular complexes (ABCs) to be formed by mixing Avidin DH (Reagent A) with its paired biotinylated enzyme (Reagent B) prior to use. The ABC reagent, once formed, remains stable for many hours after formation and can be used for several days after preparation.The VECTASTAIN® ABC Reagent can be used to detect any molecule that is biotinylated. This property gives the avidin-biotin complex (ABC) method great versatility in the types of targets that can be detected as well as the types of applications in which it can be employed. Biotinylated primary antibodies, secondaries, lectins, neuronal tracers, nucleic acids, and ligands can be effectively visualized in applications such as:Tissue stainingMultiple labeling (Multiplex IHC)Western blottingSouthern and northern blottingIn situ hybridization detection (ISH)Enzyme immunoassays (ELISA)Neuronal tracingAll applications benefit from the high sensitivity, low background, reproducibility, and economy of the VECTASTAIN® ABC system.

Universal (Mouse/Rabbit/Goat)

Immunohistochemistry / Immunocytochemistry

2° - 8°C – Do Not Freeze

Ambient

Concentrate

美国