官网链接
折扣/货期| 货号：GTX30945 | 规格：50ml | 目录价：￥1500 |
产品详情
产品名称:
TMB Substrate
产品描述:
TMB Substrate
实验应用:
ELISA
克隆号:
产品浓度:
保存温度:
Store at 2-8ºC.
运输温度:
4°C
产地:
美国
功能与背景:
3,3'5,5’- tetramethylbenzidine (TMB) is a soluble chromogen substrate for horseradish peroxidase detection systems for ELISA. This is ready to use one component system and gives soluble blue color with peroxidase which absorbs at 653 nm; on acidification gives stable yellow color that absorbs at 450 nm. The extinction coefficient of blue color ( E653 nm = 3.9 x 104 mol-1 cm-1 ), and the yellow color is ( E450 nm = 5.9 x 104 mol-1 cm-1 ). TMB yields stable blue color as end product ., that can be measured at 650 nm; the color formation is function of time The reaction is stopped by adding acid solution it yields a yellow color that can be measured at 450 nm. This yellow color is 1.5 X more sensitive than blue color. This TMB solution contains TMB, 2.08 mMol L-1, Hydrogen Peroxide substrate, citrate buffer and non-toxic proprietary stabilizers.
促销活动
