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折扣/货期| 货号：GTX78422 | 规格：100ml | 目录价：￥3800 |
产品详情
产品名称:
TMB Substrate
产品描述:
3,3′,5,5′-Tetramethylbenzidine (TMB) is a chromogenic substrate suitable for use in ELISA procedures, which utilize horseradish peroxidase conjugates. This substrate produces a soluble end product that is blue in color and can be read spectrophotometrically at 370 or 655 nm. The reaction maybe stopped with acid, resulting in a yellow solution that is read at 450 nm. This product is supplied as a ready-to-use peroxidase substrate containing TMB in a mildly acidic buffer. Prior to reaction with peroxidase, the substrate should be a colorless to light bluish-green solution. The substrate system develops a blue reaction product when reacted with peroxidase in microwell applications (e.g. ELISA assays). For end-point assays, acid can be used to stop the reaction, to yield a yellow end product. Since this substrate produces a soluble reaction product, it is not recommended for histochemistry or blotting.
实验应用:
ELISA
克隆号:
产品浓度:
保存温度:
Store at 4ºC. Protect from light.
运输温度:
4°C
产品形式:
Liquid
存储溶液:
TMB is a combination of buffer, chromogen and H₂O₂, as a substrate, in one convenient single component reagent, no preservative.
产地:
美国
功能与背景:
3-3',5,5'-tetramethylbenzidine (TMB) is a soluble chromogen substrate for horseradish peroxidase detection systems. It is recommended for ELISA procedures.
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